NMR solution structure of chitin-binding domain from dust mite group XII allergen Blo t 12
Solution structure of Blo 1 12 CBD domain.
The data deposited under this entry was used to solve structure of chitin-binding domain of allergen Blo t 12. The structure describes Blo t 12.0102 isoform identified from the mites found in Singapore.
FIRST FIVE RESIDUES (GPLGS) ARE EXPRESSION TAGS
NMR data was acquired at 295K using Shigemi NMR tubes.
Initial structure ensemble was calculated by semi-automated NOESY assignment by CYANA. The assignments were manually verified in Sparky and final structure annealing was performed in CYANA. Structure and restraints from CYANA were imported in Xplor-NIH for explicit water refinement.
torsion angle dynamics, DGSA-distance geometry simulated annealing
1 mM [U-100% 13C; U-100% 15N] Blo t 12 CBD-1, 50 mM potassium phosphate-2, 100 mM sodium chloride-3, 90% H2O/10% D2O
1 mM [U-100% 15N] Blo t 12 CBD-4, 50 mM potassium phosphate-5, 100 mM sodium chloride-6, 90% H2O/10% D2O
1 mM [U-100% 15N] Blo t 12 CBD-7, 50 mM potassium phosphate-8, 100 mM sodium chloride-9, 11 mg/ml Pf1 phage-10, 90% H2O/10% D2O
Schwieters, Kuszewski, Tjandra and Clore