Polyaminooligonucleotide: NMR structure of duplex DNA containing a nucleoside with spermine residue, N-[4,9,13-triazatridecan-1-yl]-2'-deoxycytidine.
synthesized using phosphoramidite chemistry
0.5 mM DNA (5'-D(*GP*TP*CP*GP*GP*CP*TP*G)-3'), 0.5 mM DNA (5'-D(*CP*AP*GP*CP*CP*GP*AP*C)-3'), 100 % [U-100% 2H] D2O, 50 mM sodium chloride, 10 mM sodium phosphate, 0.1 mM EDTA, 100% D2O
0.5 mM DNA (5'-D(*GP*TP*CP*GP*GP*CP*TP*G)-3'), 0.5 mM DNA (5'-D(*CP*AP*GP*CP*CP*GP*AP*C)-3'), 10 % [U-100% 2H] D2O, 90 % H2O, 50 mM sodium chloride, 10 mM sodium phosphate, 0.1 mM EDTA, 90% H2O/10% D2O
Schwieters, Kuszewski, Tjandra and Clore