K11-linked Diubiquitin average solution structure at pH 6.8, 0 mM NaCl
Unique structural, dynamical, and functional properties of k11-linked polyubiquitin chains.
K11-linked Ub2 average solution structure in the presence of NaCl
DISTAL UBIQUITIN (CHAIN A) AND PROXIMAL UBIQUITIN (CHAIN B) ARE CONNECTED VIA A COVALENT LINKAGE BETWEEN C GLY 76 OF CHAIN A AND NZ LYS 11 OF CHAIN B. THIS LINKAGE HAS NOT BEEN MODELED IN THIS STRUCTURE.
Ub/Ub orientation determined from RDCs via alignment tensors, and z-distance refined using site-directed spin labeling distance constraints (MTSL on distal position 36)
in-house RDC (orientation) and MTSL (distance)
75 uM [U-99% 15N] ubiquitin, 20 mM sodium phosphate, 93% H2O/7% D2O
Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax