Restrained CS-Rosetta Solution NMR structure of the CARDB domain of PF1109 from Pyrococcus furiosus. Northeast Structural Genomics Consortium target PfR193A
Northeast Structural Genomics Consortium (NESG)
PDBStat: a universal restraint converter and restraint analysis software package for protein NMR.
MEFPDLTVEIKGPDVVGVNKLAEYEVHVKNLGGIGVPSTKVRVYINGTLYKNWTVSLGPKEEKVLTFSWTPTQEGMYRIN ATVDEENTVVELNENNNVATFDVSVVLEHHHHHH
restrained CS-Rosetta structure refinement
Northeast Structural Genomics Consortium
CYANA/CNS-Water refined structure determination
THE FIRST REPRESENTATIVE STRUCTURE IN THE ENSEMBLE IS THE MEDOID STRUCTURE.
2D 1H-13C HSQC high resolution (L/V methyl stereoassignment)
Structures were calculated by Restrained CS-Rosetta method using NOE-based distance and dihedral angle restraints from the original CNS-water refined structure calculations
0.65 mM [U-100% 13C; U-100% 15N] PfR193A, 20 mM MES, 200 mM sodium chloride, 5 mM calcium chloride, 10 mM DTT, 0.02 % sodium azide, 50 uM DSS, 10 % D2O, 90 % H2O, 90% H2O/10% D2O
0.43 mM [U-5% 13C; U-100% 15N] PfR193A, 20 mM MES, 200 mM sodium chloride, 5 mM calcium chloride, 10 mM DTT, 0.02 % sodium azide, 50 uM DSS, 10 % D2O, 90 % H2O, 90% H2O/10% D2O
Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax
CARDB domain, PFAM 07705, UNKNOWN FUNCTION, Structural Genomics, NORTHEAST STRUCTURAL GENOMICS CONSORTIUM, NESG, PSI-Biology, Protein Structure Initiative
EFPDLTVEIKGPDVVGVNKLAEYEVHVKNLGGIGVPSTKVRVYINGTLYKNWTVSLGPKEEKVLTFNWTPTQEGMYRINA TVDEENTVVELNENNNVATFDVSVV