Blue Light-Absorbing State of TePixJ, an Active Cyanobacteriochrome Domain
Phycoviolobilin, blue light-absorbing form
3-[5-[(3-ethyl-4-methyl-5-oxidanylidene-1,2-dihydropyrrol-2-yl)methyl]-2-[[5-[(Z)-(4-ethyl-3-methyl-5-oxidanylidene-pyr rol-2-ylidene)methyl]-3-(3-hydroxy-3-oxopropyl)-4-methyl-1H-pyrrol-2-yl]methyl]-4-methyl-1H-pyrrol-3-yl]propanoic acid
Structural and Flexibility Variations Between the Ground and Photoactivated States of a Blue/Green Light-Absorbing Cyanobacteriochrome
MAAVQLSELRDRQAIFETLVAKGRELLACDRVIVYAFDDNYVGTVVAESVAEGWPQARDQVIEDPCFREHWVEAYRQGRI QATTDIFKAGLTECHLNQLRPLKVRANLVVPMVIDDQLFGLLIAHQASEPRQWQEIEIDQFSELASTGSLVLERLHFLEQ TIASL
Pb state of TePixJ(GAF) solved to 1.77 A resolution
Pb state of TePixJ(GAF) solved to 2.45 A resolution
simulated annealing, torsion angle dynamics
0.7-1.3 mM [U-13C; U-15N] TePixJ, 0.7-1.3 mM PHYCOVIOLOBILIN, 93% H2O/7% D2O
Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax
Schwieters, Kuszewski, Tjandra and Clore
phytochrome, PixJ, blue/green light-absorbing, cyanobacteria, PVB, phycoviolobilin, SIGNALING PROTEIN
AAVQLSELRDRQAIFETLVAKGRELLACDRVIVYAFDDNYVGTVVAESVAEGWPQARDQVIEDPCFREHWVEAYRQGRIQ ATTDIFKAGLTECHLNQLRPLKVRANLVVPMVIDDQLFGLLIAHQCSEPRQWQEIEIDQFSELASTGSLVLERLHFLEQT IA