Solution structure of a complex consisting of hDlg/SAP-97 residues 318-406 and HPV51 oncoprotein E6 residues 141-151
Structural insights into a wildtype domain of the oncoprotein E6 and its interaction with a PDZ domain.
Synapse-associated protein 97, SAP-97, SAP97, hDlg
MEIKLIKGPKGLGFSIAGGVGNQHIPGDNSIYVTKIIEGGAAHKDGKLQIGDKLLAVNNVCLEEVTHEEAVTALKNTSDF VYLKVAKPTGSHHHHHH
In addition to the experiments listed below, filtered/edited experiments were performed to distinguish intra- and inter-molecular NOEs
DGSA-distance geometry simulated annealing, molecular dynamics
0.8 mM [U-100% 13C; U-100% 15N] hDlgPDZ2, 2.0 mM E6CT11, 20 mM sodium phosphate, 4 mM TCEP, 0.05 w/v sodium azide, 90% H2O/10% D2O
0.8 mM [U-100% 13C; U-100% 15N] hDlgPDZ2, 2.0 mM E6CT11, 20 mM sodium phosphate, 4 mM TCEP, 0.05 w/v sodium azide, 100% D2O
3.12 mM hDlgPDZ2, 1.25 mM [U-100% 13C; U-100% 15N] E6CT11, 20 mM sodium phosphate, 4 mM TCEP, 0.05 w/v sodium azide, 100% D2O
3.12 mM hDlgPDZ2, 1.25 mM [U-100% 13C; U-100% 15N] E6CT11, 20 mM sodium phosphate, 4 mM TCEP, 0.05 w/v sodium azide, 90% H2O/10% D2O
Brunger, Adams, Clore, Gros, Nilges and Read
Papillomavirus E6 Proteins, HPV, Oncoprotein E6, E6, Viral, Oncogene Proteins, PDZ Domain, hDlgPDZ2, hDlg, hDlg1, SAP-97, Dlg, ONCOPROTEIN - CELL ADHESION protein complex, ONCOPROTEIN-CELL ADHESION complex
MEIKLIKGPKGLGFSIAGGVGNQHIPGDNSIYVTKIIEGGAAHKDGKLQIGDKLLAVNNVCLEEVTHEEAVTALKNTSDF VYLKVAKPT