African Swine Fever Virus Pol X in the ternary complex with MgdGTP and DNA
How a low-fidelity DNA polymerase chooses non-Watson-Crick from Watson-Crick incorporation.
MLTLIQGKKIVNHLRSRLAFEYNGQLIKILSKNIVAVGSLRREEKMLNDVDLLIIVPEKKLLKHVLPNIRIKGLSFSVKV CGERKCVLFIEWEKKTYQLDLFTALAEEKPYAIFHFTGPVSYLIRIRAALKKKNYKLNQYGLFKNQTLVPLKITTEKELI KELGFTYRIPKKRL
0.5 mM ILV-CH3-[U-13C; U-15N; U-2H] protein, 10 mM Mg, 4 mM dGTP, 0.6 mM [U-100% 13C; U-100% 15N] DNA, 50 mM Borate, 10 mM [U-2H] DTT, 200 mM potassium chloride, 95% H2O/5% D2O
DNA polymerase, Nucleotidyl Transferase, TRANSFERASE