Solution Structure of Helix-35 Stem-loop from E. coli 23S rRNA
RNA (5'-R(*GP*GP*GP*CP*UP*AP*AP*UP*GP*UP*UP*GP*AP*AP*AP*AP*AP*UP*UP*AP*GP*CP*CP*C)-3')
RNA was prepared by in vitro transcription
1.2 mM [U-99% 13C; U-99% 15N] RNA, 10 mM potassium phosphate, 10 mM sodium chloride, 0.1 mM EDTA, 100% D2O
1.6 mM RNA, 10 mM potassium phosphate, 10 mM sodium chloride, 0.1 mM EDTA, 100% D2O
1.6 mM RNA, 10 mM potassium phosphate, 10 mM sodium chloride, 0.1 mM EDTA, 90% H2O/10% D2O
1.2 mM [U-99% 13C; U-99% 15N] RNA, 10 mM potassium phosphate, 10 mM sodium chloride, 0.1 mM EDTA, 90% H2O/10% D2O
Schwieters, Kuszewski, Tjandra and Clore
ribosome, 23S rRNA, methylation, erythromycin resistance, RNA