Selective recruitment of an e2~ubiquitin complex by an e3 ubiquitin ligase.
Protein ZYP, RING finger protein 75, RING-box protein 1, Rbx1, Regulator of cullins 1
GGGGTNSGAGKKRFEVKKSNASAQSAWDIVVDNCAICRNHIMDLCIECQANQASATSEECTVAWGVCNHAFHFHCISRWL KTRQVCPLDNREWEFQKYGH
DGSA-distance geometry simulated annealing, torsion angle dynamics
20 mM sodium phosphate, 100 mM sodium chloride, 1 mM DTT, 700 uM [U-98% 15N] protein, 90% H2O/10% D2O
20 mM sodium phosphate, 100 mM sodium chloride, 1 mM DTT, 700 uM [U-98% 13C; U-98% 15N] protein, 90% H2O/10% D2O
20 mM sodium phosphate, 100 mM sodium chloride, 1 mM DTT, 700 uM [U-98% 13C; U-98% 15N] protein, 100% D2O
Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax
Schwieters, Kuszewski, Tjandra and Clore
Laskowski, MacArthur, Smith, Jones, Hutchinson, Morris, Moss and Tho
GTNSGAGKKRFEVKKWNAVALWAWDIVVDNCAICRNHIMDLCIECQANQASATSEECTVAWGVCNHAFHFHCISRWLKTR QVCPLDNREWEFQKYGH