Structural studies of the PARP-1 BRCT domain.
PARP-1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1, Poly[ADP-ribose] synthase 1
MKPLSNMKILTLGKLSQNKDEAKAMIEKLGGKLTGSANKASLCISTKKEVEKMSKKMEEVKAANVRVVCEDFLQDVSASA KSLQELLSAHSLSSWGAEVKHHHHHH
Final Refinement from cyana generated restraints, Multiple rounds of calculations
simulated annealing, simulated annealing
3 mM [U-100% 13C; U-100% 15N] protein, 40 mM sodium phosphate, 140 mM sodium chloride, 95% H2O/5% D2O
Schwieters, Kuszewski, Tjandra and Clore
Brunger, Adams, Clore, Gros, Nilges and Read
Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax
KPLSNMKILTLGKLSQNKDEAKAMIEKLGGKLTGSANKASLCISTKKEVEKMSKKMEEVKAANVRVVCEDFLQDVSASAK SLQELLSAHSLSSWGAEVK