A Conformational Switch in the CRIB-PDZ Module of Par-6.
GSKTKAPSISIPHDFRCVSAIIDVDIVPETHRRVRLCKHGSDKPLGFYIRDGTSVRVTASGLEKQPGIFISRLVPGGLAE STGLLAVNDEVIEVNGIEVAGKTLDQVTDMMVANSSNLIITVKPANQR
AUTOMATED METHODS WERE USED FOR BACKBONE CHEMICAL SHIFT ASSIGNMENT AND ITERATIVE NOE REFINEMENT. FINAL STRUCTURES WERE OBTAINED BY MOLECULAR DYNAMICS IN EXPLICIT SOLVENTSTRUCTURES ARE BASED ON A TOTAL OF 1604 NOE CONSTRAINTS ( 407 INTRA, 388 SEQUENTIAL, 251 MEDIUM, AND 558 LONG RANGE) AND 165 PHI AND PSI DIHEDRAL ANGLE CONSTRAINTS., STRUCTURES ARE BASED ON A TOTAL OF 1604 NOE CONSTRAINTS ( 407 INTRA, 388 SEQUENTIAL, 251 MEDIUM, AND 558 LONG RANGE) AND 165 PHI AND PSI DIHEDRAL ANGLE CONSTRAINTS.
1 mM [U-100% 13C; U-100% 15N] Par-6, 20 mM sodium phosphate, 50 mM sodium chloride, 90% H2O, 10% D2O
Eccles, C., Guntert, P., Billeter, M., Wuthrich, K.
KTKAPSISIPHDFRQVSAIIDVDIVPETHRRVRLLKHGSDKPLGFYIRDGTSVRVTASGLEKQPGIFISRLVPGGLAEST GLLAVNDEVIEVNGIEVAGKTLDQVTDMMVANSSNLIITVKPANQR