The solution structure of the HAMP domain of the hypothetical transmembrane receptor Af1503
The Mechanisms of HAMP-Mediated Signaling in Transmembrane Receptors.
The HAMP domain structure implies helix rotation in transmembrane signaling.
structures with the least restraint violations
Refinement against a conformational database potential
1 mM Af1503 HAMP, 20 mM phosphate buffer, 150 mM sodium chloride, 90% H2O/10% D2O
1 mM [U-100% 15N] Af1503 HAMP, 20 mM phosphate buffer, 150 mM sodium chloride, 90% H2O/10% D2O
0.5 mM [U-100% 13C; U-100% 15N] Af1503 HAMP, 0.5 mM Af1503 HAMP, 20 mM phosphate buffer, 150 mM sodium chloride, 90% H2O/10% D2O
Schwieters, Kuszewski, Tjandra and Clore