NMR structure of fusion of CtIP (641-685) to LMO4-LIM1 (18-82)
Structural Basis of the Interaction of the Breast Cancer Oncogene LMO4 with the Tumour Suppressor CtIP/RBBP8.
The fusion protein of CtIP (UNP 641-685), LINKER (901-911) and LMO4 (UNP 18-82)
DNA endonuclease RBBP8,LIM domain transcription factor LMO4
CtBP-interacting protein,CtIP,Retinoblastoma-binding protein 8,RBBP-8,Retinoblastoma-interacting protein and myosin-like,RIM,Sporulation in the absence of SPO11 protein 2 homolog,SAE2,Breast tumor autoantigen,LIM domain only protein 4,LMO-4
GSSLQNNQDVSFENIQWSIDPGADLSQYKMDVTVIDTKDGSQSKLGGGGSGGHMGSGGLSWKRCAGCGGKIADRFLLYAM DSYWHSRCLKCSSCQAQLGDIGTSSYTKSGMILCRNDYIRLFG
CtIP on N-terminus, followed by 11-residue linker, LMO4-LIM1
THIS FUSION PROTEIN HAS NON-SEQUENTIAL RESIDUE NUMBERING. THE RESIDUE 901 LINKS TO THE RESIDUE 685 AND THE RESIDUE 18 LINKS TO THE RESIDUE 911, RESPECTIVELY.
150 uM [U-15N] Fusion protein-1, 20 mM sodium phosphate-2, 150 mM sodium chloride-3, 1 mM DTT-4, 90% H2O/10% D2O
500 uM [U-99% 13C; U-99% 15N] Fusion protein-5, 20 mM sodium phosphate-6, 150 mM sodium chloride-7, 1 mM DTT-8, 90% H2O/10% D2O
300 uM [U-99% 13C; U-99% 15N] Fusion protein-9, 20 mM sodium phosphate-10, 150 mM sodium chloride-11, 1 mM DTT-12, 100% D2O
500 uM Fusion protein-13, 20 mM sodium phosphate-14, 150 mM sodium chloride-15, 1 mM DTT-16, 90% H2O/10% D2O
LIM domain, protein-protein interaction, LIM-interaction domain, LMO4, RBBP8/CtIP, LIM-only protein, HYDROLASE, METAL BINDING PROTEIN