The DNA-binding domain of human PARP-1 interacts with DNA single-strand breaks as a monomer through its second zinc finger.
PARP-1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1, Poly[ADP-ribose] synthase 1
MAESSDKLYRVEYAKSGRASCKKCSESIPKDSLRMAIMVQSPMFDGKVPHWYHFSCFWKVGHSIRHPDVEVDGFSELRWD DQQKVKKTAEAGGVTGKGQDGIGSKAEK
50 mM [U-99% 2H] TRIS-1, 200 mM sodium chloride-2, 150 uM zinc sulfate-3, 4 mM [U-99% 2H] DTT-4, 95% H2O/5% D2O
Schwieters, Kuszewski, Tjandra and Clore
Poly [ADP-ribose] polymerase 1 (E.C.2.4.2.30)
zinc finger, TRANSFERASE, DNA REPAIR PROTEIN