Second PBZ domain of human APLF protein in complex with ribofuranosyladenosine

Solution structures of the two PBZ domains from human APLF and their interaction with poly(ADP-ribose).

sequence database residues 363-451, PBZ-type 2 domain

Apurinic-apyrimidinic endonuclease APLF, PNK and APTX-like FHA domain-containing protein, XRCC1-interacting protein 1

GPLGSGSEGNKVKRTSCMYGANCYRKNPVHFQHFSHPGDSDYGGVQIVGQDETDDRPECPYGPSCYRKNPQHKIEYRHNT
LPVRNVLDE

FIRST PBZ DOMAIN OF HUMAN APLF PROTEIN IN COMPLEX WITH RIBOFURANOSYLADENOSINE

RESIDUES 363-404 ARE NOT SHOWN IN THE COORDINATES BECAUSE STRUCTURE CALCULATIONS WERE CARRIED OUT ON RESIDUES 405-451 ONLY.

The author states that NMR was carried out on a single fragment (363-451) containing both fingers F1 and F2 of APLF, but the structure calculations were carried out separately for each finger. This co-ordinate file includes residues 405-451 and contains F2 as well as the unstructured regions on either side.

20 mM potassium pyrophosphate, 200 mM sodium chloride, 100 uM zinc sulphate, 2 mM [U-2H] DTT, 0.8 mM [U-98% 13C; U-98% 15N] APLF_363-451, 2 mM RFA, 95% H2O/5% D2O

20 mM potassium pyrophosphate, 200 mM sodium chloride, 100 uM zinc sulphate, 2 mM [U-2H] DTT, 0.8 mM [U-98% 13C; U-98% 15N] APLF_363-451, 2 mM RFA, 100% D2O

Schwieters, Kuszewski, Tjandra and Clore

ADP-ribosylation, DNA damage, DNA repair, Metal-binding, Nucleotide-binding, Nucleus, Zinc, Zinc-finger, LYASE

GSEGNKVKRTSCMYGANCYRKNPVHFQHFSHPGDSDYGGVQIVGQDETDDRPECPYGPSCYRKNPQHKIEYRHNTLPVRN
VLDE