Solution structure of the a' domain of thermophilic fungal protein disulfide isomerase
Redox-Dependent Domain Rearrangement of Protein Disulfide Isomerase Coupled with Exposure of Its Substrate-Binding Hydrophobic Surface
GPLGSEGPVTVVVAKNYNEIVLDDTKDVLIEFYAPWCGHCKALAPKYEELGALYAKSEFKDRVVIAKVDATANDVPDEIQ GFPTIKLYPAGAKGQPVTYSGSRTVEDLIKFIAENGKYKAA
DGSA-distance geometry simulated annealing
1.0mM [U-13C; U-15N] PDI A'-1, 10mM sodium phosphate-2, 100mM potassium chloride-3, 10mM DTT-4, 90% H2O/10% D2O
1.0mM [U-15N] PDI A'-5, 10mM sodium phosphate-6, 100mM potassium chloride-7, 10mM DTT-8, 99% D2O
Brunger, Adams, Clore, Gros, Nilges, Read
THIOREDOXIN FOLD, ISOMERASE, Disulfide bond, Endoplasmic reticulum, Redox-active center
EGPVTVVVAKNYNEIVLDDTKDVLIEFYAPWCGHCKALAPKYEELGALYAKSEFKDRVVIAKVDATANDVPDEIQGFPTI KLYPAGAKGQPVTYSGSRTVEDLIKFIAENGKYKAA