NMR solution structures of 3-hydroxyoctanoyl-ACP from Streptomyces coelicolor Fatty Acid Synthase
S-[2-[3-[[(2S)-2-hydroxy-3,3-dimethyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3R)-3-hydroxyoctanethioate
Recognition of intermediate functionality by acyl carrier protein over a complete cycle of fatty acid biosynthesis
AATQEEIVAGLAEIVNEIAGIPVEDVKLDKSFTDDLDVDSLSMVEVVVAAEERFDVKIPDDDVKNLKTVGDATKYILDHQ A
20 best structures from last iteration were selected for water refinement. Water refined structures were calculated using the slightly modified refinement script applied to the RECOORD database.
10mM potassium phosphate-1, 0.5 mM sodium azide-2, 1mM 3-hydroxyoctanoyl phosphopantetheine-3, 1mM [U-99% 13C; U-99% 15N] ACP-4, 95% H2O/5% D2O
10mM potassium phosphate-5, 0.5mM sodium azide-6, 1mM 3-hydroxyoctanoyl phosphopantetheine-7, 1mM [U-99% 13C; U-99% 15N] ACP-8, 95% H2O/5% D2O
Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax
acyl carrier protein, intermediate binding, fatty acid synthase, Fatty acid biosynthesis, Lipid synthesis, Phosphopantetheine, TRANSPORT PROTEIN