Use of Relaxation Enhancements in a Paramagnetic Environment for the Structure Determination of Proteins Using NMR Spectroscopy
PERE structure of maltose-binding protein
PERE restraints were incorporated using lab-written scripts
simulated annealing, Paramagnetic environment relaxation enhancement refinement
50mM potassium phosphate-1, 3mM sodium azide-2, 90% H2O/10% D2O
Brunger, Adams, Clore, Gros, Nilges and Read
ubiquitin, paramagnetic relaxation, Gd(DTPA-BMA), Cytoplasm, Isopeptide bond, Nucleus, Phosphoprotein, Ubl conjugation, SIGNALING PROTEIN