Solution Structure of C-terminal domain of oxidized NleG2-3 (residue 90-191) from Pathogenic E. coli O157:H7. Northeast Structural Genomics Consortium and Midwest Center for Structural Genomics target ET109A
Northeast Structural Genomics Consortium (NESG)
Midwest Center for Structural Genomics (MCSG)
Ontario Centre for Structural Proteomics (OCSP)
NleG Type 3 effectors from enterohaemorrhagic Escherichia coli are U-Box E3 ubiquitin ligases.
A novel strategy for NMR resonance assignment and protein structure determination.
SQESIQNKISQCKFSVCPERLQCPLEAIQCPITLEQPEKGIFVKNSDGSDVCTLFDAAAFSRLVGEGLPHPLTREPITAS IIVKHEECIYDDTRGNFIIKGN
Northeast Structural Genomics Consortium
Ontario Centre for Structural Proteomics
0.5 mM [U-100% 13C; U-100% 15N] NleG, 10 mM [U-100% 2H] TRIS, 300 mM sodium chloride, 10 mM DTT, 1 mM benzamidine, 0.01 % sodium azide, 90% H2O/10% D2O
0.5 mM [U-7% 13C; U-100% 15N] NleG, 10 mM [U-100% 2H] TRIS, 300 mM sodium chloride, 10 mM DTT, 1 mM benzamidine, 0.01 % sodium azide, 90% H2O/10% D2O
Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax
Brunger, Adams, Clore, Gros, Nilges and Read
Methods Development, U-box domain, Structural Genomics, PSI-2, Protein Structure Initiative, Northeast Structural Genomics Consortium, NESG, Midwest Center for Structural Genomics, MCSG, Ontario Centre for Structural Proteomics, OCSP, UNKNOWN FUNCTION