Solution structure of MLL CXXC domain in complex with palindromic CPG DNA
Structure of the MLL CXXC domain-DNA complex and its functional role in MLL-AF9 leukemia.
Zinc finger protein HRX, ALL-1, Trithorax-like protein, Lysine N-methyltransferase 2A, CXXC-type zinc finger protein 7
ALL1, CXXC7, HRX, HTRX, KMT2A, MLL, TRX1
DNA (5'-D(*DCP*DCP*DCP*DTP*DGP*DCP*DGP*DCP*DAP*DGP*DGP*DG)-3')-2
DNA (5'-D(*DCP*DCP*DCP*DTP*DGP*DCP*DGP*DCP*DAP*DGP*DGP*DG)-3')-4
DNA (5'-D(*DCP*DCP*DCP*DTP*DGP*DCP*DGP*DCP*DAP*DGP*DGP*DG)-3')-6
DNA (5'-D(*DCP*DCP*DCP*DTP*DGP*DCP*DGP*DCP*DAP*DGP*DGP*DG)-3')-8
HIGH TEMP ANNEALING WITHOUT RRDCS, LOW TEMP ANNEALING WITH RDCS
1 mM [U-98% 13C; U-98% 15N] CXXC domain-1, 1 mM DNA (5'-D(*DCP*DCP*DCP*DTP*DGP*DCP*DGP*DCP*DAP*DGP*DGP*DG)-3')-2, 95% H2O/5% D2O
1 mM [U-98% 13C; U-98% 15N] CXXC domain-3, 1 mM DNA (5'-D(*DCP*DCP*DCP*DTP*DGP*DCP*DGP*DCP*DAP*DGP*DGP*DG)-3')-4, 100% D2O
1 mM [U-98% 13C; U-98% 15N] CXXC domain-5, 1 mM DNA (5'-D(*DCP*DCP*DCP*DTP*DGP*DCP*DGP*DCP*DAP*DGP*DGP*DG)-3')-6, 95% H2O/5% D2O
1 mM [U-98% 15N] CXXC domain-7, 1 mM DNA (5'-D(*DCP*DCP*DCP*DTP*DGP*DCP*DGP*DCP*DAP*DGP*DGP*DG)-3')-8, 100% D2O
Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax
Brunger, Adams, Clore, Gros, Nilges and Read
PROTEIN-DNA COMPLEX, CXXC DOMAIN, MLL, CPG DNA, CHROMOSOMAL REARRANGEMENT, DNA-BINDING, METAL-BINDING, NUCLEUS, ZINC-FINGER, DNA BINDING PROTEIN-DNA COMPLEX, Alternative splicing, Apoptosis, Bromodomain, Chromatin regulator, Isopeptide bond, Methyltransferase, Phosphoprotein, Polymorphism, Proto-oncogene, S-adenosyl-L-methionine, Transcription, Transcription regulation, Transferase, Ubl conjugation, Zinc