Solution structure of the extracellular domain of JTB
The structure of the extracellular domain of the jumping translocation breakpoint protein reveals a variation of the midkine fold.
Jumping translocation breakpoint protein
extracellular domain (UNP residues 47-104)
Protein JTB, Prostate androgen-regulated protein, PAR protein
Distance restraints were derived from 15N- or 13C-resolved 3D NOESY experiments and a 2D homonuclear 1H NOESY experiment. Restraints for the backbone angles phi and psi were derived from 3J(HN,Ha) coupling constants and TALOS. Stereospecific assignments of Leu, Val methyl groups were obtained using a 10% fractionally 13C-labeled sample. For the definition of H-bonds, slowly exchanging amide protons were identified from 1H, 15N correlation experiments after redissolving lyophilized protein in D2O.
1 mM [U-100% 15N] JTB, 50 mM sodium phosphate, 150 mM sodium chloride, 0.1 mM sodium azide, 90% H2O/10% D2O
1 mM [U-100% 13C; U-100% 15N] JTB, 50 mM sodium phosphate, 150 mM sodium chloride, 0.1 mM sodium azide, 90% H2O/10% D2O
1 mM JTB, 50 mM sodium phosphate, 150 mM sodium chloride, 0.1 mM sodium azide, 100% D2O
Brunger, Adams, Clore, Gros, Nilges and Read
cysteine-rich domain, Membrane, Transmembrane, MEMBRANE PROTEIN