KLENOW FRAGMENT WITH BRIDGING-SULFUR SUBSTRATE AND MANGANESE

2'-DEOXY-3'-THIOURIDINE 5'-(DIHYDROGEN PHOSPHATE)

Structures of normal single-stranded DNA and deoxyribo-3'-S-phosphorothiolates bound to the 3'-5' exonucleolytic active site of DNA polymerase I from Escherichia coli.

Structural Principles for the Inhibition of the 3'-5' Exonuclease Activity of Escherichia Coli DNA Polymerase I by Phosphorothioates

MISYDNYVTILDEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDAPDQISRERALE
LLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILNSVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQ
LTFNQIALEEAGRYAAEDADVTLQLHLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRL
AELEKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLPKVILEYRGLAKLKSTYTDK
LPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVRNEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGL
LTAFAEGKDIHRATAAEVFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGVLEYME
RTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADIIKRAMIAVDAWLQAEQPRVRMIMQVHDE
LVFEVHKDDVDAVAKQIHQLMENCTRLDVPLLVEVGSGENWDQAH

STRUCTURE WAS SOLVED USING IN-HOUSE HIGH-RESOLUTION STRUCTURE

ATOM O3* OF RESIDUE 1006B HAS BEEN REPLACED BY THE HETEROATOM S 1006

BULK-SOLVENT CORRECTION WAS USED TO EXTEND LOW-RES.LIMIT TO 20 A

ONLY LAST THREE NUCLEOTIDES OF NUCLEIC ACID COULD BE MODELED INTO ELECTRON DENSITY.

COMPLEX (POLYMERASE-DNA), EXONUCLEASE, TRANSFERASE, TRANSFERASE-DNA COMPLEX