Monomeric structure of the cardioprotective chemokine SDF-1/CXCL12
SDF-1, C-X-C motif chemokine 12, Pre-B cell growth-stimulating factor, PBSF, hIRH, SDF-1-alpha(3-67)
AUTOMATED METHODS WERE USED FOR BACKBONE CHEMICAL SHIFT ASSIGNMENT AND ITERATIVE NOE REFINEMENT. FINAL STRUCTURES WERE OBTAINED BY MOLECULAR DYNAMICS IN EXPLICIT SOLVENT, CXCL12 STRUCTURES ARE BASED ON A TOTAL OF 1140 NOE CONSTRAINTS ( 393 INTRA, 239 SEQUENTIAL, 168 MEDIUM, 340 LONG RANGE) AND 50 PHI AND PSI DIHEDRAL ANGLE CONSTRAINTS.
1.2 mM CXCL12/SDF1-alpha, 90% H2O, 10% D2O
Schwieters, Kuszewski, Tjandra and Clore
Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax
stromal cell derived factor-1, SDF1-alpha, CXCL12, chemokine, Alternative splicing, Chemotaxis, Cytokine, Growth factor, Secreted