Solution NMR structure of trans-4-hydroxynonenal derived dG adduct of (6R,8S,11R)-configuration opposite dC
The stereochemistry of trans-4-hydroxynonenal-derived exocyclic 1,N2-2'-deoxyguanosine adducts modulates formation of interstrand cross-links in the 5'-CpG-3' sequence.
trans-4-hydroxynonenal derived dG adduct of (6S,8R,11S)-configuration matched with dC
back calculated data agree with experimental NOESY spectrum
DNA (5'-(*DGP*DCP*DTP*DAP*DGP*DCP*DGP*DAP*DGP*DTP*DCP*DC)-3')
DNA (5'-(*DGP*DGP*DAP*DCP*DTP*DCP*DGP*DCP*DTP*DAP*DGP*DC)-3')
1 mM DNA (5'-D(*DGP*DCP*DTP*DAP*DGP*DCP*DGP*DAP*DGP*DTP*DCP*DC)-3'), 1 mM DNA (5'-D(*DGP*DGP*DAP*DCP*DTP*DCP*DGP*DCP*DTP*DAP*DGP*DC)-3'), 100% D2O
Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm
HNE-dG (6R, 8S, 11R), HNE derived dG adduct, trans-4-hydroxynonenal, DNA