Solution Structure of the IsdC NEAT domain bound to Zinc Protoporphyrin

The IsdC Protein from Staphylococcus aureus Uses a Flexible Binding Pocket to Capture Heme.

Iron-regulated surface determinant protein C

MGSSHHHHHHSSGLVPRGSHMSANAADSGTLNYEVYKYNTNDTSIANDYFNKPAKYIKKNGKLYVQITVNHSHWITGMSI
EGHKENIISKNTAKDERTSEFEVSKLNGKIDGKIDVYIDEKVNGKPFKYDHHYNITYKFNGPTDVAG

50 mM sodium phosphate, 100 mM sodium chloride, 0.01 % sodium azide, 1.3 mM [U-100% 13C; U-100% 15N] IsdC, 2.1 mM PROTOPORPHYRIN IX CONTAINING ZN, 7 % [U-100% 2H] D2O, 93% H2O/7% D2O

50 mM sodium phosphate, 100 mM sodium chloride, 0.01 % sodium azide, 1.1 mM IsdC, 2.3 mM PROTOPORPHYRIN IX CONTAINING ZN, 100 % D2O, 100% D2O

Schwieters, Kuszewski, Tjandra and Clore

Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax

NEAT domain, NMR complex, heme, Isd, IsdC, Cell wall, Iron, Metal-binding, Peptidoglycan-anchor, Secreted, TRANSPORT PROTEIN, HEME-BINDING PROTEIN

SANAADSGTLNYEVYKYNTNDTSIANDYFNKPAKYIKKNGKLYVQITVNHSHWITGMSIEGHKENIISKNTAKDERTSEF
EVSKLNGKIDGKIDVYIDEKVNGKPFKYDHHYNITYKFNGPTDVAG