Solid-State NMR Structure of Microcrystalline Ubiquitin
Protein structure determination from 13C spin-diffusion solid-state NMR spectroscopy.
Protein Structure Determination from 13C Spin-Diffusion Solid-State NMR
Proton Driven Spin-Diffusion experiments were carried out using a triple 2.5-mm Chemagnetics/Varian T3 probe at a static magnetic field of 14.09 T. Three 2D 13C-13C correlation spectra have been recorded with 100, 250 and 400 ms mixing time at 12 kHz magic angle spinning (MAS) and a sample temperature of 269 K.
OPALp, Luginbuhl, P. et al., J. Biomol. NMR 1996,8,(2),136-146; Konradi, R. et al., Comput. Phys. Commun. 2000, 124, 139-147.
5.5 mg/mL [U-99% 13C; U-98% 15N] Ubiquitin, 20 mM ammonium acetate, 20 mM citric acid, 60 v/v 2-methyl-2,4-pentandiol, 0.05 % sodium azide, 100 % H2O
Luginbuhl, Guntert, Billeter and Wuthrich
Protein Microcrystal, Cytoplasm, Ubl conjugation, SIGNALING PROTEIN