Structure of the calcineurin-NFAT complex

Structure of the Calcineurin-NFAT Complex: Defining a T Cell Activation Switch Using Solution NMR and Crystal Coordinates.

Calmodulin-dependent calcineurin A subunit alpha isoform

Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform, CAM-PRP catalytic subunit

VPFPPSHRLTAKEVFDNDGKPRVDILKAHLMKEGRLEESVALRIITEGASILRQEKNLLDIDAPVTVCGDIHGQFFDLMK
LFEVGGSPANTRYLFLGDYVDRGYFSIECVLYLWALKILYPKTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYDACMD
AFDCLPLAALMNQQFLCVHGGLSPEINTLDDIRKLDRFKEPPAYGPMCDILWSDPLEDFGNEKTQEHFTHNTVRGCSYFY
SYPAVCEFLQHNNLLSILRAHEAQDAGYRMYRKSQTTGFPSLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHP
YWLPNFM

The author states that this is an artificial sequence based on the NFAT sequences. The reference for the NFAT sequences is Aramburu, J., Yaffe, M. B., Lopez-Rodriguez, C., Cantley, L.C., Hogan, P.G., and Rao, A. (1999). Affinity-driven peptide selection of an NFAT inhibitor more selective than cyclosporin A. Science 285, 2129-2133.

300uM calcineurin, 0.3 mM [U-13C; U-15N; U-2H] NFAT, 90% H2O/10% D2O

400uM [2H, FIMY-1H] calcineurin, 0.4 mM [ul-2H15N, Id1LdVg -1H13C] NFAT, 100% D2O

calcineurin, NFAT, complex structure, phosphatase, HYDROLASE