Crystal structure of E.coli MenD, 2-succinyl-5-enolpyruvyl-6-hydroxy- 3-cyclohexadiene-1-carboxylate synthase - native protein

Specificity and Reactivity in Menaquinone Biosynthesis: The Structure of Escherichia Coli Mend (2-Succinyl-5-Enolpyruvyl-6-Hydroxy-3-Cyclohexadiene-1-Carboxylate Synthase).

2-SUCCINYL-5-ENOLPYRUVYL-6-HYDROXY-3-CYCLOHEXENE -1-CARBOXYLATE SYNTHASE

2-SUCCINYL-5-ENOLPYRUVYL-6-HYDROXY-3-CYCLOHEXADIENE-1-CARBOXYLATE SYNTHASE, MENAQUINONE BIOSYNTHESIS PROTEIN MEND

MGSSHHHHHHSSGENLYFQGHMSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAENSAFIHHTHFDERGLG
HLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLILLTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRP
TQDIPARWLVSTIDHALGTLHAGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWRQ
KRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSELQQAQIVVQLGSSLTGKRLLQWQA
SCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWLELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRICDY
LPEQGQLFVGNSLVVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLNALALLRQVS
APLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKYHRPQNWQELETAFADAWRTPTTTVIEMVVND
TDGAQTLQQLLAQVSHL

100 MM SODIUM CITRATE PH 5.6, 8 % ISO-PROPANOL AND 14 % PEG 6K

CRYSTAL STRUCTURE OF E.COLI MEND, 2- SUCCINYL-5-ENOLPYRUVYL-6-HYDROXY-3- CYCLOHEXADIENE-1-CARBOXYLATE SYNTHASE - SEMET PROTEIN

SEQUENCE INCLUDES N-TERMINAL HIS TAG AND TEV CLEAVAGE SITE

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.

MENAQUINONE BIOSYNTHESIS, MANGANESE, TRANSFERASE, THIAMINE DIPHOSPHATE COFACTOR, THIAMINE PYROPHOSPHATE