Biological and Structural Features of Murine Angiogenin-4, an Angiogenic Protein

MTMSPCPLLLVFVLGLVVIPPTLAQNERYEKFLRQHYDAKPQGRDDRYCESMMKERKLTSPCKDVNTFIHGTKKNIRAIC
GKKGSPYGENFRISNSPFQITTCTHSRGSPWPPCGYRAFKDFRYIVIACEDGWPVHFDESFISP

INITIAL, TWINNED CRYSTALS WERE GROWN BY MIXING EQUAL VOLUMES OF PROTEIN SOLUTION (8 MG ML IN WATER) AND RESERVOIR (25 % PEG 3350, 0.2 M LI2SO4, 0.1 M TRIS-HCL, PH 8.5). THESE CRYSTALS WERE USED TO SEED PREEQUILIBRATED DROPS CONTAINING 4 MG ML PROTEIN, 20 % PEG 3350, 0.05 M LI2SO4, 0.09 M TRIS-HCL, PH 8.5.

RESIDUES A1, A3, A50, A87, A96, B1, B53, B58, B59 TRUNCATED TO ALA DUE TO POOR DENSITY

ANGIOGENESIS, ENDONUCLEASE, DIFFERENTIATION, CANCER, NUCLEASE, HYDROLASE, RIBONUCLEASE, DEVELOPMENTAL PROTEIN, PROTEIN SYNTHESIS INHIBITOR, PYRROLIDONE CARBOXYLIC ACID