Structure of alkaline phosphatase from the Antarctic bacterium TAB5

Crystal Structure of Alkaline Phosphatase from the Antarctic Bacterium Tab5.

COVALENT LINK BETWEEN P ATOM AND S84 IN THE ACTIVE SITE

MKLKKIVFTLIALGLFSCKTTSVLVKNEPQLKTPKNVILLISDGAGLSQISSTFYFKEGTPNYTQFKNIGLIKTSSSRED
VTDSASGATAFSCGIKTYNAAIGVADDSTAVKSIVEIAALNNIKTGVVATSSITHATPASFYAHALNRGLEEEIAMDMTE
SDLDFFAGGGLNYFTSRKDKKDVLAILKGNQFTINTTGLTDFSSIASNRKMGFLLADEAMPTMESGRGNFLSAATDLAIQ
FLSKDNSAFFIMSEGSQIDWGGHANNASYLISEINDFDDAIGTALAFAKKDGNTLVIVTSDHETGGFTLAAKKNKREDGS
EYSDYTSIGPSFSTGGHSATLIPVFAYGPGSEEFIGIYENNEIFHKILKVTKWNQ

MKLKKIVFTLIALGLFSCKTTSVLVKNEPQLKTPKNVILLISDGAGLSQISSTFYFKSGTPNYTQFKNIGLIKTSSSRED
VTDSASGATAFSCGIKTYNAAIGVADDSTAVKSIVEIAALNSIKTGVVATSSITHATPASFYAHALNRGLEEEIAMDMTE
SDLDFFAGGGLNYFTSRSDSKDVLAILKGNQFTINTTGLTDFSSIASNRKMGFLLADEAMPTMEKGRGNFLSAATDLAIQ
FLSKDNSAFFIMSEGSQIDWGGHANNASYLISEINDFDDAIGTALAFAKSDGNTLVIVTSDHETGGFTLAAKSNKREDGS
EYSDYTEIGPTFSTGGHSATLIPVFAYGPGSEEFIGIYENNEIFHKILKVTKWNQ

HANGING-DROP, 4 DEGREE C, 17 MG/ML PROTEIN, 23% PEG3350, 0.2M NAAC, 10MM 4-NITROPHENYL PHOSPHATE, 0.1M CACODYLATE, PH 6.5

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. 5-6 SOME RESIDUES IN CROWN DOMAIN AND 8-10 AT N-TERMINUS HAS NOT BEEN MODELLED DUE TO MISSING ELECTRON DENSITY IN THIS REGIONS.

HYDROLASE, ALKALINE PHOSPHATASE, COLD ADAPTATION, PSYCROPHILES