Crystal Structure of Escherichia coli RNase T

Crystal Structure of RNase T, an Exoribonuclease Involved in tRNA Maturation and End Turnover.

(MSE)SDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITLK(MSE)DEQGWL(MSE)PDTTLHFHVEPFVGA
NLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKVVRKGIKASGCNRAI(MSE)VAHNANFDHSF(MSE)(MSE)AAAERA
SLKRNPFHPFATFDTAALAGLALGQTVLSKACQTAG(MSE)DFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLSAA
EEV

MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITLKMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGI
DPNDPDRGAVSEYEALHEIFKVVRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLSAAEEV

2.3 M ammonium sulfate, 0.1 M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Orthologous protein from a different organism and described in the same publication.

The biological assembly of RNase T is a homodimer. The four subunits in this structure form two homodimers: chains A and B form one homodimer; chains C and D form another homodimer.

RNase, ribonuclease, exoribonuclease, exonuclease, nuclease, hydrolase, stable RNA maturation, tRNA end-turnover