Crystal structure of the adenine-specific DNA methyltransferase M.TaqI complexed with the cofactor analog AETA and a 10 bp DNA containing an abasic site analog at the target base partner position

Influence of the target base partner on the methylation rate of the adenine-specific DNA methyltransferase M.TaqI

Structure of the N6-adenine DNA methyltransferase M.TaqI in complex with DNA and a cofactor analog

Differential binding of s-adenosylmethionine, s-adenosylhomocysteine and sinefungin to the adenine-specific DNA methyltransferase M.TaqI

DNA partner strand containing N6-methyladenine and an abasic site analog at the target base partner position

Adenine-specific methyltransferase TaqI, M.TaqI

MGLPPLLSLPSNSAPRSLGRVETPPEVVDFMVSLAEAPRGGRVLEPACAHGPFLRAFREAHGTAYRFVGVEIDPKALDLP
PWAEGILADFLLWEPGEAFDLILGNPPYGIVGEASKYPIHVFKAVKDLYKKAFSTWKGKYNLYGAFLEKAVRLLKPGGVL
VFVVPATWLVLEDFALLREFLAREGKTSVYYLGEVFPQKKVSAVVIRFQKSGKGLSLWDTQESESGFTPILWAEYPHWEG
EIIRFETEETRKLEISGMPLGDLFHIRFAARSPEFKKHPAVRKEPGPGLVPVLTGRNLKPGWVDYEKNHSGLWMPKERAK
ELRDFYATPHLVVAHTKGTRVVAAWDERAYPWREEFHLLPKEGVRLDPSSLVQWLNSEAMQKHVRTLYRDFVPHLTLRML
ERLPVRREYGFHTSPESARNF

3 microliters crystallization buffer (10 mM Tris/HCl, 300 mM NaCl, pH 7.3) containing the complex plus 1 microliter reservoir solution (100 mM KCl, 100 mM MgCl2, 6% isopropanol, 50 mM sodium cacodylate, pH 6.0), VAPOR DIFFUSION, HANGING DROP, temperature 298K

adenine-specific DNA methyltransferase M.TaqI/DNA/AETA COMPLEX

adenine-specific DNA methyltransferase M.TaqI/SAM complex

adenine-specific DNA methyltransferase M.TaqI/SAH complex

adenine-specific DNA methyltransferase M.TaqI/sinefungin complex

adenine-specific DNA methyltransferase M.TaqI/2PR-DNA/AETA COMPLEX

adenine-specific DNA methyltransferase M.TaqI/2PR-3DR-DNA/AETA COMPLEX

ADENINE-SPECIFIC DNA METHYLTRANSFERASE M.TAQI/5PY-DNA/AETA COMPLEX

ADENINE-SPECIFIC DNA METHYLTRANSFERASE M.TAQI/4PC-DNA/AETA COMPLEX

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

The asymmetric unit contains one independent biological assembly consisting of M.TaqI, DNA and cofactor analog AETA.

DNA, DNA methyltransferase, target base partner, abasic site analog, base flipping, nucleotide flipping, TRANSFERASE-DNA COMPLEX