ANISOTROPIC STRUCTURE OF PROTEIN G IGG-BINDING DOMAIN III AT 1.1 ANGSTROM RESOLUTION
Anisotropic Refinement of a Protein G Domain at 1.1 Angstrom Resolution
The Third Igg-Binding Domain from Streptococcal Protein G. An Analysis by X-Ray Crystallography of the Structure Alone and in a Complex with Fab
EXTENSION OF FORMER ISOTROPIC MODEL TO ANISOTROPIC MODEL
CRYSTALS WERE GROWN BY HANGING DROP VAPOUR DIFFUSION FROM 24-26% PEG 4000, 10MM SODIUM ACETATE AT PH 4.8 AND 0.01% SODIUM AZIDE. CELL PARAMETERS ARE NOT THOSE DETERMINED EXPERIMENTALLY. THEY WERE ADJUSTED ON THE BASIS OF THE ENGH & HUBER DICTIONARY AT THE END OF REFINEMENT. THE EXPERIMENTAL ESTIMATES WERE KNOWN TO HAVE POTENTIAL ERRORS DUE TO INACCURACIES IN THE CRYSTAL-TO-DETECTOR AND WAVELENGTH CALIBRATION., vapor diffusion - hanging drop
PROLSQ AND SHELX-93 ALSO USED IN PRELIMINARY STAGES. SHELX-93 AND SHELX-96 REFINEMENT IS AGAINST INTENSITIES.
INITIAL MODEL WAS PREVIOUSLY OBTAINED STRUCTURE
ATOMIC RESOLUTION, PROTEIN G, IMMUNOGLOBULIN-BINDING, IGG-BINDING PROTEIN, TRANSMEMBRANE
MEKEKKVKYFLRKSAFGLASVSAAFLVGSTVFAVDSPIEDTPIIRNGGELTNLLGNSETTLALRNEESATADLTAAAVAD TVAAAAAENAGAAAWEAAAAADALAKAKADALKEFNKYGVSDYYKNLINNAKTVEGIKDLQAQVVESAKKARISEATDGL SDFLKSQTPAEDTVKSIELAEAKVLANRELDKYGVSDYHKNLINNAKTVEGVKELIDEILAALPKTDTYKLILNGKTLKG ETTTEAVDAATAEKVFKQYANDNGVDGEWTYDDATKTFTVTEKPEVIDASELTPAVTTYKLVINGKTLKGETTTKAVDAE TAEKAFKQYANDNGVDGVWTYDDATKTFTVTEMVTEVPGDAPTEPEKPEASIPLVPLTPATPIAKDDAKKDDTKKEDAKK PEAKKDDAKKAETLPTTGEGSNPFFTAAALAVMAGAGALAVASKRKED