Crystal structure of Human Gem G-domain bound to GDP

Structure-function studies of the G-domain from human gem, a novel small G-protein.

GTP-binding mitogen-induced T-cell protein, RAS-like protein KIR

GSGNTYYRVVLIGEQGVGKSTLANIFAGVHDSMDSDCEVLGEDTYERTLMVDGESATIILLDMWENKGENEWLHDHCMQV
GDAYLIVYSITDRASFEKASELRIQLRRARQTEDIPIILVGNKSDLVRCREVSVSEGRACAVVFDCKFIETSAAVQHNVK
ELFEGIVRQVRLRR

20% PEG 8000, 0.1 M HEPES, 0.1 M sodium chloride, 0.5 mM magnesium chloride, 1 mM DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS