CRYSTAL STRUCTURE OF THE E267R MUTANT OF A HALOPHILIC MALATE DEHYDROGENASE IN THE APO FORM

Halophilic adaptation: novel solvent protein interactions observed in the 2.9 and 2.6 A resolution structures of the wild type and a mutant of malate dehydrogenase from Haloarcula marismortui.

New York : Cold Spring Harbor Laboratory Press
Protocol 21: The MPD-NaCl-H2O System for the Crystallization of Halophilic Proteins

Structural Features Stabilizing Halophilic Malate Dehydrogenase from an Archaebacterium

A Single Amino Acid Mutation Enhances the Halophilic Behaviour of Malate Dehydrogenase from Haloarcula marismortui

Cloning, Sequencing, and Expression in Escherichia coli of the Gene Coding for Malate Dehydrogenase of the Extremely Halophilic Archaebacterium Haloarcula marismortui

TKVSVVGAAGTVGAAAGYNIALRDIADEVVFVDIPDKEDDTVGQAADTNHGIAYDSNTRVRQGGYEDTAGSDVVVITAGI
PRQPGQTRIDLAGDNAPIMEDIQSSLDEHNDDYISLTTSNPVDLLNRHLYEAGDRSREQVIGFGGRLDSARFRYVLSEEF
DAPVQNVEGTILGEHGDAQVPVFSKVRVDGTDPEFSGDEKEQLLGDLQESAMDVIERKGATEWGPARGVAHMVEAILHDT
GRVLPASVKLEGEFGHEDTAFGVPVRLGSNGVEEIVEWDLDDYEQDLMADAAEKLSDQYDKIS

THREE KINDS OF COMPLEX SALT BRIDGES MAY BE DESCRIBED INCLUDING: CL, LYS A 205, ASP D 211, ARG D 207, GLU A 188, GLU D 188, ARG A 207, ASP A 211, LYS D 203, CL, AND ITS SYMMETRY RELATED; ASP A 209, ARG D 292, GLU D 299 AND ITS 3 OTHERS SYMMETRY RELATED; LYS A 243, GLU A 247, NA+, GLU B 247, LYS B 243, AND ITS SYMMETRY RELATED.

HALOPHILIC, ION-BINDING, SALT BRIDGES, MALATE DEHYDROGENASE, OXIDOREDUCTASE