Structure of Zeta-Zeta Transmembrane Dimer
The structure of the zetazeta transmembrane dimer reveals features essential for its assembly with the T cell receptor.
T-cell surface glycoprotein CD3 zeta chain
standard HSQC and trosy-HSQC for D(NH) couplings; 2D (CA)CONH quantitative JCH experiment for D(CaHa) couplings
426 intramolecular NOE restraints, 46 intermolecular NOE restraints, 42 dihedral angle restraints, 70 residual dipolar coupling restraints.
0.5-1.0 mM disulfide crosslinked zeta-zeta dimer U-15N,U-13C,85%-2H. Sodium dodecyl sulfate (40 mM) and dodecyl phosphocholine (200 mM), 95% H2O, 5% D2O.
0.5-1.0 mM disulfide crosslinked zeta-zeta dimer U-15N,U-13C. Sodium dodecyl sulfate (40 mM) and dodecyl phosphocholine (200 mM), 95% H2O, 5% D2O.
0.5-1.0 mM disulfide crosslinked zeta-zeta dimer, mixed-label. One monomer: U-15N,U-2H. Other monomer: natural abundance isotopes. Sodium dodecyl sulfate (40 mM) and dodecyl phosphocholine (200 mM), 95% H2O, 5% D2O.
0.5-1.0 mM disulfide crosslinked zeta-zeta dimer U-15N,U-13C. Sodium dodecyl sulfate (40 mM) and dodecyl phosphocholine (200 mM), 95% H2O, 5% D2O. 5% polyacrylamide gel.
transmembrane, alpha helix, MEMBRANE PROTEIN