Solution structure of the talin F3 domain in complex with a chimeric beta3 integrin-PIP kinase peptide
Structural basis of integrin activation by talin
Chimera of 24-mer peptide from Integrin beta-3 and 10-mer peptide from Phosphatidylinositol-4-phosphate 5-kinase type-1 gamma
Chimera of beta3-integrin and PIP-kinase
PLGSGVSFFLVKEKMKGKNKLVPRLLGITKESVMRVDEKTKEVIQEWSLTNIKRWAASPKSFTLDFGDYQDGYYSVQTTE GEQIAQLIAGYIDIILKKKKS
minimized average structure of the same protein
The structures are based on a total of 2175 restraints, of which 2015 are NOE-derived distance restraints, 126 dihedral angle restraints and 34 distance restraints from hydrogen bonds.
1.2mM Talin F3 U-15N; 1.4mM chimeric peptide; 50mM phosphate buffer pH 6.1, 100mM NaCl; 95% H2O, 5% D2O
1.5mM Talin F3; 1.5mM chimeric peptide; 50mM phosphate buffer pH 6.1, 100mM NaCl; 99.96% D2O
1.9mM Talin F3 U-15N,2H; 1.5mM chimeric peptide; 50mM phosphate buffer pH 6.1, 100mM NaCl; 95% H2O, 5% D2O
1.9mM Talin F3 U-15N,2H; 1.5mM chimeric peptide; 50mM phosphate buffer pH 6.1, 100mM NaCl; 99.96% D2O
0.9mM Talin F3 U-15N,13C; 1.1mM chimeric peptide; 50mM phosphate buffer pH 6.1, 100mM NaCl; 95% H2O, 5% D2O
Protein-peptide complex, alpha helix, beta sheet, STRUCTURAL PROTEIN
GVSFFLVKEKMKGKNKLVPRLLGITKECVMRVDEKTKEVIQEWSLTNIKRWAASPKSFTLDFGDYQDGYYSVQTTEGEQI AQLIAGYIDIILKKKKS