Protein kinase A fivefold mutant model of Rho-kinase with Y-27632

(R)-TRANS-4-(1-AMINOETHYL)-N-(4-PYRIDYL) CYCLOHEXANECARBOXAMIDE

Structural analysis of protein kinase A mutants with Rho-kinase inhibitor specificity

Protein kinase A in complex with Rho-kinase inhibitors Y-27632, Fasudil, and H-1152P: structural basis of selectivity

cAMP-dependent protein kinase, alpha-catalytic subunit

cAMP-dependent protein kinase inhibitor alpha

PKI-alpha, cAMP-dependent protein kinase inhibitor, muscle/brain isoform

GNAAAAKKGSEQESVKEFLAKAKEDFLKKWENPAQNTAHLDQFERIKTIGTGSFGRVMLVKHMETGNHYAMKILDKQKVV
KLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMVMEYMPGGDMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSL
DLIYRDLKPENLLIDQQGYIKVADFGFAKRVKGRTW(TPO)LCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYP
PFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFATTDWIAIYQRKVEAPFIP
KFKGPGDTSNFDDYEEEEIRV(SEP)INEKCGKEFSEF

GNAAAAKKGSEQESVKEFLAKAKEDFLKKWENPAQNTAHLDQFERIKTIGTGSFGRVMLVKHMETGNHYAMKILDKQKVV
KLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMVMEYMPGGDMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSL
DLIYRDLKPENLLIDQQGYIKVADFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFA
DQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFATTDWIAIYQRKVEAPFIPKFKG
PGDTSNFDDYEEEEIRVSINEKCGKEFSEF

Methanol, LiCl, MesBisTris, EDTA, DTT, Mega8, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 278K

PKA five fold mutant model of Rho-kinase

PKA five fold mutant model of Rho-kinase with H1152P

PKA five fold mutant model of Rho-kinase with inhibitor Fasudil (HA1077)

PKA three fold mutant model of Rho-kinase with Y-27632

PKA three fold mutant model of Rho-kinase with inhibitor H-1152P

The peptide was chemically synthesized. The sequence of the peptide is naturally found in Rabbit.

an unpublished structure of the binary complex of PKA C alpha wildtype enzyme with PKI(5-24)

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

PKA, Rho-kinase, surrogate, Y-27632, TRANSFERASE-TRANSFERASE INHIBITOR COMPLEX

GNAAAAKKGSEQESVKEFLAKAKEDFLKKWENPAQNTAHLDQFERIKTLGTGSFGRVMLVKHMETGNHYAMKILDKQKVV
KLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMVMEYVPGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSL
DLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFA
DQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFATTDWIAIYQRKVEAPFIPKFKG
PGDTSNFDDYEEEEIRVSINEKCGKEFSEF