Solution structure of the Brinker DNA binding domain in complex with the omb enhancer
DNA recognition by the brinker repressor - an extreme case of coupling between binding and folding
Brinker DNA binding domain (residues 43-101)
This structure was determined using standard 2D and 3D heteronuclear techniques and 12C/14N-filtered-NOESY experiments for DNA assignment and intermolecular NOE contacts
The structures are based on 1279 NOE-derived distance constraints, 21 Ambiguous Interaction Restraints, 21 distance restraints from hydrogen bonds, 92 RDC restraints, 30 dihedral angle restraints, 56 J-coupling constant restraints.
torsion angle dynamics; distance geometry
1mM brkDBD U-15N, 1mM DNA omb12T5 NA; 10mM NaCl; 5mM potassium phosphate; 5mM TCEP; 0.02% NaN3; 95% H2O, 5% D2O
1mM brkDBD U-13C/15N, 1mM DNA omb12T5 NA; 10mM NaCl; 5mM potassium phosphate; 5mM TCEP; 0.02% NaN3; 95% H2O, 5% D2O
0.35mM brkDBD U-13C/15N, 0.35mM DNA omb12 NA; 10mM NaCl; 20mM potassium phosphate; 2mM DTT; 0.02% NaN3; 25mg/ml Pf1-phages; 95% H2O, 5% D2O
PROTEIN-DNA COMPLEX, HELIX-TURN-HELIX MOTIF, TRANSCRIPTION-DNA COMPLEX