Complex of TM1a(1-14)Zip with TM9a(251-284): a model for the polymerization domain ("overlap region") of tropomyosin, Northeast Structural Genomics Target OR9
Northeast Structural Genomics Consortium (NESG)
Solution NMR Structure of the Junction between Tropomyosin Molecules: Implications for Actin Binding and Regulation.
This peptide is the product of a synthetic gene. It contains GCG at the N terminus followed by residues 251-284 of rat striated tropomyosin. The peptide has the mutation N279K
This peptide is the product of a synthetic gene. It contains a gly at the N terminus followed by the first 14 residues of rat striated tropomyosin and the last 18 residues of yeast GCN4
Tropomyosin 1 alpha chain/General control protein GCN4
Rattus norvegicus, Saccharomyces cerevisiae
pSBETc, pET3a, and pET11a cut with NdeI and BamHI
Northeast Structural Genomics Consortium
Structure of a two-chain coiled coil containing the first 14 residues of the N terminus of muscle tropomyosins and the last 18 residues of the C terminus of the yeast transcription GCN4. The peptide is acetylated at the N terminus.
Two-chain coiled-coil synthetic molecule containing residues 251-284 of the C terminus of tropomyosin with GlyCysGly are at the N-terminus. The cystines are linked by a disulfide.
10 structures from initial DYANA calcultions with the lowest target functions were refined using CNS. The structures back calculated data agree with experimental NOESY spectra. The structures have acceptable covalent geometry, favorable non-bond energy, the lowest energy and the fewest restraint violations.
The structures were based on a total of 2630 restraints, 2198 conformationally restricting NOEs, 232 dihedral angle constraints and 200 hydrogen bond constraints
Initail structure was calculated with Torsion Angle Dynamics and refined with simulated annealing and included a term for explicit solvent in the refinement protocol.
1mM complex of TM1a(1-14)Zip U-15N withTM9a(251-284)U15N, 100 mM NaCl, 10 mM sodium phospate 10% deuterium oxide pH 6.5
100 mM NaCl, 10 mM sodium phospate 10% deuterium oxide pH 6.5
1mM complex of TM1a(1-14)Zip U15N/U13C with TM9a(251-284) unlabled, 100 mM NaCl, 10 mM sodium phospate 10% deuterium oxide pH 6.5
1mM complex of TM1a(1-14)Zip U15N/U13C with TM9a(251-284) unlabled, 100 mM NaCl, 10 mM sodium phospate 99.9% deuterium oxide pH 6.5
100 mM NaCl, 10 mM sodium phospate 99.9% deuterium oxide pH 6.5
1mM complex of TM1a(1-14)Zip unlabeled with TM9a(251-284)U15N/U13C, 100 mM NaCl, 10 mM sodium phospate 10% deuterium oxide pH 6.5 or in 99.9% deuterium oxide
100 mM NaCl, 10 mM sodium phospate 10% deuterium oxide pH 6.5 or in 99.9% deuterium oxide
1mM complex of TM1a(1-14)Zip unlabeled with TM9a(251-284)U15N/U13C, 100 mM NaCl, 10 mM sodium phospate 10% deuterium oxide 99.9% deuterium oxide, pH 6.5
100 mM NaCl, 10 mM sodium phospate 10% deuterium oxide 99.9% deuterium oxide, pH 6.5
1mM complex of TM1a(1-14), one chain labeled U15N/U13C the other chain unlabeled, with unlabeled TM9a(251-284), 100 mM NaCl, 10 mM sodium phospate 10% deuterium oxide pH 6.5
1mM complex of TM9a(251-284), one chain labeled U15N/U13C the other chain unlabeled, with unlabele TM1a(1-14)Zip, 100 mM NaCl, 10 mM sodium phospate 10% deuterium oxide pH 6.5
TROPOMYOSIN, PEPTIDE COMPLEX, OVERLAP COMPLEX, INTERMOLECULAR JUNCTION, N-TERMINAL:C-TERMINAL INTERFACE, PARALLEL COILED COIL, POLYMERIZATION DOMAIN, STRUCTURAL PROTEIN, Structural Genomics, PSI-2, Protein Structure Initiative, Northeast Structural Genomics Consortium, NESG