Crystal Structure of Gyp1 TBC domain in complex with Rab33 GTPase bound to GDP and AlF3

TBC-domain GAPs for Rab GTPases accelerate GTP hydrolysis by a dual-finger mechanism.

MGHHHHHHGSLVPRGSDVQELNSIIQRISKFDNILKDKTIINQQDLRQISWNGIPKIHRPVVWKLLIGYLPVNTKRQEGF
LQRKRKEYRDSLKHTFSDQHSRDIPTWHQIEIDIPRTNPHIPLYQFKSVQNSLQRILYLWAIRHPASGYVQGINDLVTPF
FETFLTEYLPPSQIDDVKIKDPSTYMVDEQITDLEADTFWCLTKLLEQITDNYIHGQPGILRQVKNLSQLVKRIDADLYN
HFQNEHVEFIQFAFRWMNCLLMREFQMGTVIRMWDTYLSETSQEVTSSYSMSSNDIKPPVTPTEPRVASFVTPTKDFQSP
TTALSNMTPNNAVEDSGKMRQSSLNEFHVFVCAAFLIKWSDQLMEMDFQETITFLQNPPTKDWTETDIEMLLSEAFIWQS
LYKDATSHWL

MGHHHHHHGSSSCAVSGASGCLPPARSRIFKIIVIGDSNVGKTCLTYRFCAGRFPDRTEATIGVDFRERAVDIDGERIKI
QLWDTAGQERFRKSMVQHYYRNVHAVVFVYD(MSE)TN(MSE)ASFHSLPAWIEECKQHLLANDIPRILVGNKCDLRSAI
QVPTDLAQKFADTHS(MSE)PLFETSAKNPNDNDHVEAIF(MSE)TLAHKLKSH

MGHHHHHHGSSSCAVSGASGCLPPARSRIFKIIVIGDSNVGKTCLTYRFCAGRFPDRTEATIGVDFRERAVDIDGERIKI
QLWDTAGQERFRKSMVQHYYRNVHAVVFVYDMTNMASFHSLPAWIEECKQHLLANDIPRILVGNKCDLRSAIQVPTDLAQ
KFADTHSMPLFETSAKNPNDNDHVEAIFMTLAHKLKSH

10~15% polyethylene glycol 6000, 100 mM Sodium Acetate, 10% Glycerol, 20 mM NaF, 2mM AlCl3, 50 mM HEPES, pH7.0, EVAPORATION, temperature 277K

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

biological unit is same as protein complex in the asymmetric unit

Protein transport, Gyp1 TBC domain, Rab33, vesicular trafficking, HYDROLASE ACTIVATOR-PROTEIN TRANSPORT COMPLEX