The crystal structure of ribD from Escherichia coli

The Crystal Structure of the Bifunctional Deaminase/Reductase RibD of the Riboflavin Biosynthetic Pathway in Escherichia coli: Implications for the Reductive Mechanism.

MDYKDDDDKGSSTSLYKKAGSETLYIQGQDEYY(MSE)ARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQRAGEP
HAEVHALR(MSE)AGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAS(MSE)QDPNPQVAGRGLYRLQQAGID
VSHGL(MSE)(MSE)SEAEQLNKGFLKR(MSE)RTGFPYIQLKLGASLDGRTA(MSE)ASGESQWITSPQARRDVQLLRA
QSHAILTSSATVLADDPALTVRWSELDEQTQALYPQQNLRQPIRIVIDSQNRVTPVHRIVQQPGETWFARTQEDSREWPE
TVRTLLIPEHKGHLDLVVL(MSE)(MSE)QLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLCTLP
GLEKLADAPQFKFKEIRHVGPDVCLHLVGASTHHHHHH

MDYKDDDDKGSSTSLYKKAGSETLYIQGQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQRAGEPHAEV
HALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQDPNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQ
LNKGFLKRMRTGFPYIQLKLGASLDGRTAMASGESQWITSPQARRDVQLLRAQSHAILTSSATVLADDPALTVRWSELDE
QTQALYPQQNLRQPIRIVIDSQNRVTPVHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHKGHLDLVVLMMQLGKQQI
NSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLCTLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGASTHHHH
HH

In 500uL reservoir: 0.1M MES, 3% (v/v) 1,6 Hexandiol, 2+2uL drops , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

Riboflavin biosynthesis protein ribD (E.C.1.1.1.193, 3.5.4.26)

ribD apo structure, Structural Genomics, Structural Proteomics in Europe, SPINE, HYDROLASE, OXIDOREDUCTASE

QDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQRAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRT
PPCCDALIAAGVARVVASMQDPNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGRT
AMASGESQWITSPQARRDVQLLRAQSHAILTSSATVLADDPALTVRWSELDEQTQALYPQQNLRQPIRIVIDSQNRVTPV
HRIVQQPGETWFARTQEDSREWPETVRTLLIPEHKGHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYI
APKLLGSDARGLCTLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA