structure of vSET in complex with meK27 H3 Pept. and cofactor product SAH

Structural insights of the specificity and catalysis of a viral histone H3 lysine 27 methyltransferase.

chirality errors at SER23,ASN56,LEU62,SER216.

PBCV-1 histone H3-Lys 27 methyltransferase

MFNDRVIVKKSPLGGYGVFARKSFEKGELVEECLCIVRHNDDWGTALEDYLFSRKNMSAMALGFGAIFNHSKDPNARHEL
TAGLKRMRIFTIKPIAIGEEITISYGDDYWLSRPRLTQN

The structure was determined using triple-resonance NMR spectroscopy.

simulated annealing
torsion angle dynamics

0.5 mM protein with 2.5 mM meK27 H3 Pept. and 2.5mM SAH
in a 50 mM phosphate buffer of pH 6.5 containing 700 mM NaCl, 300 mM urea, 0.1 mM EDTA and 5 mM -ME in H2O/2H2O (9/1) or 2H2O

vSET structure, hsitone methyltransferase, TRANSFERASE