catalytic domain of bovine beta 1, 4-galactosyltransferase in complex with alpha-lactalbumin, glucose, Mn, and UDP-N-acetylgalactosamine

N-acetyl-beta-D-galactosamine; 2-acetamido-2-deoxy-beta-D-galactose; 2-acetamido-2-deoxy-D-galactose; 2-acetamido-2-deoxy-galactose; N-ACETYL-D-GALACTOSAMINE

Structural Snapshots of beta-1,4-Galactosyltransferase-I Along the Kinetic Pathway.

TELTKCKVSHAIKDIDGYQGISLLEWACVLFHTSGYDTQAVVNDNGSTEYGLFQISDRFWCKSSEFPESENICGISCDKL
LDDELDDDIACAKKILAIKGIDYWKAYKPMCSEKLEQWRCEKP

ASMTGGQQMGRGSSLTACPEESPLLVGPMLIEFNIPVDLKLVEQQNPKVKLGGRYTPMDCISPHKVAIIIPFRNRQEHLK
YWLYYLHPILQRQQLDYGIYVINQAGESMFNRAKLLNVGFKEALKDYDYNCFVFSDVDLIPMNDHNTYRCFSQPRHISVA
MDKFGFSLPYVQYFGGVSALSKQQFLSINGFPNNYCGWGGEDDDIYNRLAFRGMSVSRPNAVIGKTRMIRHSRDKKNEPN
PQRFDRIAHTKETMLSDGLNSLTYMVLEVQRYPLYTKITVDIGTCS

50 mM Mes-NaOH, 6% PEG 4000, pH 6.5, VAPOR DIFFUSION, temperature 298K

Crystal structure of a 1:1 complex between alpha-lactalbumin and beta 1, 4-galactosyltransferase in the presence of UDP-N-acetyl-galactosamine

Although intact UDP-GalNAc was used in the crystallization, in the crystal structure the GalNAc moiety is cleaved off from UDP-GalNAc. This cleaved off sugar exists without O1 oxygen atom similar to an oxocarbenium ion or a N-acetylgalactal form, which cannot be distinguished at the present resolution

lactose sythase, catalytic intermediate, TRANSFERASE

SLTACPEESPLLVGPMLIEFNIPVDLKLVEQQNPKVKLGGRYTPMDCISPHKVAIIIPFRNRQEHLKYWLYYLHPILQRQ
QLDYGIYVINQAGESMFNRAKLLNVGFKEALKDYDYNCFVFSDVDLIPMNDHNTYRCFSQPRHISVAMDKFGFSLPYVQY
FGGVSALSKQQFLSINGFPNNYWGWGGEDDDIYNRLAFRGMSVSRPNAVIGKCRMIRHSRDKKNEPNPQRFDRIAHTKET
MLSDGLNSLTYMVLEVQRYPLYTKITVDIGTPS