The fibrillar tip complex of the Afa/Dr adhesins from pathogen E. coli displays synergistic binding to 5 1 and v 3 integrins
The solution structure of the invasive tip complex from Afa/Dr fibrils
MRGSHHHHHHGSAELHLESRGGSGTQLRDGAKVATGRIICREAHTGFHVWMNERQVDGRAERYVVQSKDGRHELRVRTGG DGWSPVKGEGGKGVSRPGQEEQVFFDVMADGNQDIAPGEYRFSVGGACVVPQEDNKQGFTPSGTTGTTKLTVT
Structure based on 3839 noe-based restraints (2951 unambiguous, 1423 ambiguous) and 116 torsion angles (talos)
Aria-implemented distance geometry, simulated annealing
0.5mM afaD-dsc, U-15N, 13C; 50mM Na phosphate buffer(pH 7.0); 50mM NaCl; 90% H2O, 10% D2O
0.5mM afaDE-dsc, U-15N, 13C, D2O; 50mM Na phosphate buffer(pH 7.0); 50mM NaCl; 90% H2O, 10% D2O
Delaglio, Grzesiek, Vuister, Zhu, Pfeifer, Bax
afaD, afaE, fibrillar, afimbrial, integrin-binding, DAEC, DAF, CEACAM, CELL ADHESION
AELHLESRGGSGTQLRDGAKVATGRIICREAHTGFHVWMNERQVDGRAERYVVQSKDGRHELRVRTGGDGWSPVKGEGGK GVSRPGQEEQVFFDVMADGNQDIAPGEYRFSVGGACVVPQE