Structure, dynamics, and stability variation in bacterial albumin binding modules: implications for species specificity.
Finegoldia, Streptococcus, Streptococcus, Streptococcus, Streptococcus
Finegoldia magna ATCC 29328, Streptococcus dysgalactiae, Streptococcus equi, Streptococcus canis, Streptococcus sp.
SEQUENCE The protein was obtained by recombining seven artificial constructs that cumulatively represented much of the genetic diversity found in 16 native albumin binding domains from six proteins and bacterial species namely Peptostreptococcus magnus [L3316-GA1], Streptococcus dysgalactiae [MAG-GA1], Streptococcus equi [ZAG-GA], Streptococcus canis [DG12-GA2], (Rozak, Alexander, et al. Biochemistry 2006, in press). The characters enclosed in square brackets refer to the particular albumin binding domain found in the bacteria. There is no amino acid sequence database reference available for the recombinant protein.
The structure was calculated using 1063 restraints. Model 21 is an average of the 20 conformers calculated from experimental restraints.
0.2~0.4 mM PSD-1, 50 mM sodium phosphate buffer, pH 7.0, 0.1 mM EDTA, 10% D2O, 90% H2O
solution structure, PSD-1, PROTEIN BINDING