Fitting of the gp10 trimer structure into the cryoEM map of the bacteriophage T4 baseplate in the hexagonal conformation.

Evolution of bacteriophage tails: structure of t4 gene product 10

METHOD--laplacian filtered real space REFINEMENT PROTOCOL--rigid body
correlation coefficient as defined by the program COLORES/SITUS

THE COORDINATES CONTAIN CA ONLY. RESIDUES 161-405 ARE MISSING. PLEASE SEE THE RELATED EMDB ENTRY FOR DETAILS OF THE EM RECONSTRUCTION.

The trimeric gene product 10 is a structural component of the sixfold-symmetric T4 baseplate. The entry contains the fit of the gp10 C-terminal domain crystal structure (residues 406-602) and the homology model of the gp10 N-terminal domain (residues 1-160) into the cryoEM map of the T4 baseplate in the hexagonal conformation (EMDB ID 1048). The homology model of the N-terminal domains is based on the crystal structure of T4 gp9 (PDB ID 1S2E).

MKQNINIGNVVDDGTGDYLRKGGIKINENFDELYYELGDGDVPYSAGAWKTYNASSGQTLTAEWGKSYAINTSSGRVTIN
LPKGTVNDYNKVIRARDVFATWNVNPVTLVAASGDTIKGSAVPVEINVRFSDLELVYCAPGRWEYVKNKQIDKITSSDIS
NVARKEFLVEVQGQTDFLDVFRGTSYNVNNIRVKHRGNELYYGDVFSENSDFGSPGENEGELVPLDGFNIRLRQPCNIGD
TVQIETFMDGVSQWRSSYTRRQIRLLDSKLTSKTSLEGSIYVTDLSTMKSIPFSAFGLIPGEPINPNSLEVRFNGILQEL
AGTVGMPLFHCVGADSDDEVECSVLGGTWEQSHTDYSVETDENGIPEILHFDSVFEHGDIINITWFNNDLGTLLTKDEII
DETDNLYVSQGPGVDISGDVNLTDFDKIGWPNVEAVQSYQREFNAVSNIFDTIYPIGTIYENAVNPNNPVTYMGFGSWKL
FGQGKVLVGWNEDISDPNFALNNNDLDSGGNPSHTAGGTGGSTSVTLENTNLPATETDEEVLIVDENGSVIVGGCQYDPD
ESGPIYTKYREAKASTNSTHTPPTSITNIQPYITVYRWIRIA

Fitting of the gp10 trimer structure into the cryoEM map of the bacteriophage T4 baseplate in the star conformation.

Crystal structure of the gp10 C-terminal domain

Crystal structure of T4 gene product 9 that has been used to model the N-terminal domain of gp10 (residues 1-160)

CryoEM reconstruction of the bacteriophage T4 baseplate-tail tube assembly in the hexagonal conformation

BIOMOLECULE: 1

ASSEMBLY COMPONENTS
COM_ID: 1
NAME:THE TRIMERIC GENE PRODUCT 10 IS A STRUCTURAL
COMPONENT OF THE SIXFOLD-SYMMETRIC T4 BASEPLATE
IPR_ID: NULL
GO_ID: NULL
OTHER_DETAILS: THE ENTRY CONTAINS THE FIT OF THE GP10 C-
TERMINAL DOMAIN CRYSTAL STRUCTURE (RESIDUES 406-602) AND
THE HOMOLOGY MODEL OF THE GP10 N-TERMINAL DOMAIN (RESIDUES
1-160) INTO THE CRYOEM MAP OF THE T4 BASEPLATE IN THE
HEXAGONAL CONFORMATION (EMDB ID 1048). THE HOMOLOGY MODEL
OF THE N-TERMINAL DOMAINS IS BASED ON THE CRYSTAL
STRUCTURE OF T4 GP9 (PDB ID 1S2E).

VIRUS PARTICULARS
COM_ID: 1
VIRUS_HOST_CATEGORY: BACTERIA
VIRUS_HOST_SPECIES: ESCHERICHIA COLI
VIRUS_HOST_GROWTH_CELL: ESCHERICHIA COLI
VIRUS_TYPE: VIRION
VIRUS_ISOLATE: STRAIN
ICTVDB_ID: 02.043.0.01.005

THE COORDINATES CONTAIN CA ONLY. RESIDUES 161-405 ARE MISSING.
Bacteriophage T4; Baseplate; Tail; Evolution; gp10; Structural comparisons, VIRUS-VIRAL PROTEIN COMPLEX

MKQNINIGNVVDDGTGDYLRKGGIKINENFDELYYELGDGDVPYSAGAWKTYNASSGQTLTAEWGKSYAINTSSGRVTIN
LPKGTVNDYNKVIRARDVFATWNVNPVTLVAASGDTIKGSAVPVEINVRFSDLELVYCAPGRWEYVKNKQIDKITSSDIS
NVARKEFLVEVQGQTDFLDVFRGTSYNVNNIRVKHRGNELYYGDVFSENSDFGSPGENEGELVPLDGFNIRLRQPCNIGD
TVQIETFMDGVSQWRSSYTRRQIRLLDSKLTSKTSLEGSIYVTDLSTMKSIPFSAFGLIPGEPINPNSLEVRFNGILQEL
AGTVGMPLFHCVGADSDDEVECSVLGGTWEQSHTDYSVETDENGIPEILHFDSVFEHGDIINITWFNNDLGTLLTKDEII
DETDNLYVSQGPGVDISGDVNLTDFDKIGWPNVEAVQSYQRAFNAVSNIFDTIYPIGTIYENAVNPNNPVTYMGFGSWKL
FGQGKVLVGWNEDISDPNFALNNNDLDSGGNPSHTAGGTGGSTSVTLENANLPATETDEEVLIVDENGSVIVGGCQYDPD
ESGPIYTKYREAKASTNSTHTPPTSITNIQPYITVYRWIRIA