MAD analysis of FHIT, a putative human tumor suppressor from the HIT protein family.
SELENOMETHIONYL FHIT WITH BOUND SULFATE IN ACTIVE SITE
MSFRFGQHLIKPSVVFLKTELSFALVNRKPVVPGHVLVCPLRPVERFHDLRPDEVADLFQTTQRVGTVVEKHFHGTSLTF S(MSE)QDGPEAGQTVKHVHVHVLPRKAGDFHRNDSIYEELQKHDKEDFPASWRSEEE(MSE)AAEAAALRVYFQ
MSFRFGQHLIKPSVVFLKTELSFALVNRKPVVPGHVLVCPLRPVERFHDLRPDEVADLFQTTQRVGTVVEKHFHGTSLTF SMQDGPEAGQTVKHVHVHVLPRKAGDFHRNDSIYEELQKHDKEDFPASWRSEEEMAAEAAALRVYFQ
EXPRESSED AS FUSION PROTEIN WITH GLUTATHIONE-S-TRANSFERASE
DATA WERE COLLECTED USING 0.2 DEGREE OSCILLATIONS BY CONTINUOUSLY ADDING PARTIALS.
1.25-1.35M AMSO4, 100MM SODIUM CACODYLATE PH6.5
FHIT, FRAGILE HISTIDINE TRIAD PROTEIN, PUTATIVE HUMAN TUMOR SUPPRESSOR, ADVANCED PHOTON SOURCE, APS, HIT PROTEIN FAMILY, PKCI, CHROMOSOMAL TRANSLOCATION