The crystal structure of the HlyB-NBD E631Q mutant in complex with ADP

A structural analysis of asymmetry required for catalytic activity of an ABC-ATPase domain dimer.

Alpha-hemolysin translocation ATP-binding protein hlyB

HHHHHHDITFRNIRFRYKPDSPVILDNINLSIKQGEVIGIVGRSGSGKSTLTKLIQRFYIPENGQVLIDGHDLALADPNW
LRRQVGVVLQDNVLLNRSIIDNISLANPGMSVEKVIYAAKLAGAHDFISELREGYNTIVGEQGAGLSGGQRQRIAIARAL
VNNPKILIFDQATSALDYESEHVIMRNMHKICKGRTVIIIAHRLSTVKNADRIIVMEKGKIVEQGKHKELLSEPESLYSY
LYQLQSD

100 mM Tris, 16% PEG 6000, 5% MPD, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

ABC-transporter, ATPase, TRANSPORT PROTEIN