NMR Solution Structure of protein from Zebra Fish Dr.13312
Center for Eukaryotic Structural Genomics (CESG)
GHHHHHHLEDPSGGTPYIGSKISLISKAEIRYEGILYTIDTENSTVALAKVRSFGTEDRPTDRPIAPRDETFEYIIFRGS DIKDLTVCEPPKPIM
WHEAT GERM CELL-FREE, IN VITRO EXPRESSION
Center for Eukaryotic Structural Genomics
ALL TRIPLE-RESONANCE AND NOESY SPECTRA WERE ACQUIRED USING A CRYOGENIC PROBE.
STRUCTURES ARE BASED ON A TOTAL OF 1106 NOE RESTRAINTS (974 UNAMBIGUOUS, 132 AMBIGUOUS), 40 HBOND RESTRAINTS, AND 172 PHI AND PSI DIHEDRAL ANGLE CONSTRAINTS.
simulated annealing, torsion angle dynamics, water refinement
0.46 MM PROTEIN, 100 MM NACL, 10 MM BIS-TRIS, 5 MM DTT, 90% H2O, 10% D2O
Dr.13312, BC055387, AAH55387, LSm14_N, RAP55, Sm-like protein, strongly bent five-stranded antiparallel beta-sheet, Structural Genomics, PSI, Protein Structure Initiative, Center for Eukaryotic Structural Genomics, CESG, UNKNOWN FUNCTION
SGGTPYIGSKISLISKAEIRYEGILYTIDTENSTVALAKVRSFGTEDRPTDRPIAPRDETFEYIIFRGSDIKDLTVCEPP KPIM